cloning hairpin targeting lcn2  (Addgene inc)

Journal: Oncology Reports

Article Title: High expression of eIF4A1 promotes angiogenesis through the NF-κB/VEGFA pathway and predicts poor prognosis in gastric cancer

doi: 10.3892/or.2025.8951

Figure Lengend Snippet: eIF4A1 is upregulated in gastric cancer tissues. (A) Pan-cancer analysis of eIF4A1 expression was performed using UALCAN. (B) Expression of eIF4A1 in gastric cancer tissues and in peripheral normal tissues from patients with gastric cancer was assessed using Home For Researchers database. (C) Expression of eIF4A1 in late-stage GC tissues, early-stage GC tissues and in peripheral normal tissues. (D) Statistics of eIF4A1 expression in different types of gastric tissues. (E) eIF4A1 protein in GC tissues and normal gastric tissues: (a) Well-differentiated adenocarcinoma, (b) papillary adenocarcinoma, (c) poorly-differentiated adenocarcinoma, (d) mucinous adenocarcinoma, (e) CG, (f) pericarcinomatous mucosa tissue, (g) IM and (h) HGIN. *P<0.05, ***P<0.001, ****P<0.0001. CG, chronic gastritis; eIF4A1, eukaryotic translation initiation factor 4A; HGIN, high-grade intraepithelial neoplasia; IM, intestinal metaplasia; LGIN, low-grade intraepithelial neoplasia.

Article Snippet: Lentiviruses containing eIF4A1 cDNA (pLV3-CMV-eIF4A1-CopGFP-Puro) or short hairpin (sh)RNA against eIF4A1 (pLV3-U6-eIF4A1-shRNA-CopGFP-Puro) were produced by GeneCopoeia, Inc, as well as their controls (NC and shNC).

Techniques: Expressing

Journal: Oncology Reports

Article Title: High expression of eIF4A1 promotes angiogenesis through the NF-κB/VEGFA pathway and predicts poor prognosis in gastric cancer

doi: 10.3892/or.2025.8951

Figure Lengend Snippet: Survival curves confirming the prognostic value of eIF4A1 and TNM stage in GC. (A) High eIF4A1 expression was associated with overall survival in patients with GC. (B) TNM stage was associated with the overall survival of patients with GC. GC, gastric cancer; TNM, Tumor-Node-Metastasis.

Article Snippet: Lentiviruses containing eIF4A1 cDNA (pLV3-CMV-eIF4A1-CopGFP-Puro) or short hairpin (sh)RNA against eIF4A1 (pLV3-U6-eIF4A1-shRNA-CopGFP-Puro) were produced by GeneCopoeia, Inc, as well as their controls (NC and shNC).

Techniques: Expressing

Journal: Oncology Reports

Article Title: High expression of eIF4A1 promotes angiogenesis through the NF-κB/VEGFA pathway and predicts poor prognosis in gastric cancer

doi: 10.3892/or.2025.8951

Figure Lengend Snippet: Dysregulation of eIF4A1 affects the angiogenic activity of gastric cancer cells. (A) Western blotting detected the expression of eIF4A1 in cells after lentivirus infection. (B) Cell Counting Kit 8 assay assessed the proliferation of HUVECs treated with CM derived from specific cells. The optical density at 0 h was set at 100%. (C) Representative images (left) and quantification (right) of wound healing assay of HUVECs treated with CM derived from specific cells. (D) Representative images (left) and quantification (right) of Transwell assay of HUVECs treated with CM derived from specific cells. (E) Representative images (left) and quantification (right) of angiogenesis assay of HUVECs treated with CM derived from specific cells. (F) Representative image of the tumors. (G) Growth curves of the tumors and (H) weight of the tumors. (I) Representative images (left) and quantification (right) of CD31 staining in tissues from a subcutaneous xenograft tumor model. # P<0.05 vs. NC; & P<0.05 vs. shNC; *P<0.05. CM, conditioned media; eIF4A1, eukaryotic translation initiation factor 4A; HUVECs, human umbilical cord endothelial cells; MVD, microvessel density; NC, negative control; sh, short hairpin.

Article Snippet: Lentiviruses containing eIF4A1 cDNA (pLV3-CMV-eIF4A1-CopGFP-Puro) or short hairpin (sh)RNA against eIF4A1 (pLV3-U6-eIF4A1-shRNA-CopGFP-Puro) were produced by GeneCopoeia, Inc, as well as their controls (NC and shNC).

Techniques: Activity Assay, Western Blot, Expressing, Infection, Cell Counting, Derivative Assay, Wound Healing Assay, Transwell Assay, Angiogenesis Assay, Staining, Negative Control

Journal: Oncology Reports

Article Title: High expression of eIF4A1 promotes angiogenesis through the NF-κB/VEGFA pathway and predicts poor prognosis in gastric cancer

doi: 10.3892/or.2025.8951

Figure Lengend Snippet: eIF4A1 affects regulation of the TME and angiogenesis. Association between eIF4A1 expression and various component cells in the TME was detected using R package (4.3.3) in (A) GSE62254 , (B) GSE15459 and (C) GSE84426 . Part of the left panel was enlarged and presented in the right panel. (D) Correlations between eIF4A1 expression and CD31, VEGFA, NFKB1 and NFKB2. (E) mRNA levels of VEGF family genes were detected by reverse transcription-quantitative polymerase chain reaction. (F) Changes in VEGEF and NF-κB expression after eIF4A1 overexpression and knockdown were detected by western blotting. *P<0.05. eIF4A1, eukaryotic translation initiation factor 4A; NC, negative control; sh, short hairpin; TME, tumor microenvironment. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

Article Snippet: Lentiviruses containing eIF4A1 cDNA (pLV3-CMV-eIF4A1-CopGFP-Puro) or short hairpin (sh)RNA against eIF4A1 (pLV3-U6-eIF4A1-shRNA-CopGFP-Puro) were produced by GeneCopoeia, Inc, as well as their controls (NC and shNC).

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Over Expression, Knockdown, Western Blot, Negative Control